Use of Sporekill Spot in a Fingerprint Lecanicillium Test
Report By Ralph Noble and Andreja Dobrovin-Pennington Microbiotech Ltd, Pershore Centre, Avonbank, Pershore, Worcestershire, WR10 3JP January 2021
Aim of test To compare the efficacy of Sporekill Spot (alcohol version) with an alcohol-based hand sanitizer gel in killing a Lecanicillium inoculum in a fingerprint test Materials and methods
Alcohol-based hand sanitizer gel (80% ethanol; Flu Supplies, Bath, UK) was used for comparison in the tests. Fingers were dipped into a Lecanicillium culture plate, and then into water, alcohol-based gel or Sporekill Spot (alcohol version) for 5 sec, held in a clean dry air stream for 10 sec, and then touched on to the surface of a PDA plate. The dipping procedure was repeated three times on each plate and there were three replicate plates of each treatment. The plates were incubated at 20C and observed for regrowth of Lecanicillium for up to 14 days. The plates were photographed after 4 and 7 days.
Result Mycelium of Lecanicillium was observed in all of the water treatment fingerprints on the culture plates. None of the alcohol sanitizer gel or Sporekill Spot treatment fingerprints resulted in regrowth of Lecanicillium (Figure 1).
Conclusion Sporekill Spot (alcohol version) and an alcohol-based sanitizer gel were equally effective in preventing regrowth of Lecanicillium in a fingerprint test.